Welcome!

This is the repository of photos taken by microscopy students at James Madison University. Each week, they will learn a little more about microscopy and post a new photo. At the end of semester, each student will enter their favorite image into a competition. The winner will be decided by popular vote.
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Sunday, November 27, 2011

Creative Writing

Graphite on paper was imaged using red fluorescent light on the Nikon inverted microscope at 20x magnification.

Thursday, November 17, 2011

A DIC image of a diatom at 40x with its long axis parallel to the sheer. The image was taken using Nikon Inverted microsocpe.
This is a DIC image of a diatom at 40x objective with its long axis oriented perpendicular to the sheer. Image was taken using the Nikon Inverted microscope.
This is a confocal image of BPAE cells stained with three fluoresence dye. The image was taken with the 40x objective using the Nikon Inverted Microscope. The dyes were DAPI(blue), BODIPY(green) and Texas Red(red).
Confocal image of Arabidopsis root at 40x objective using the Nikon Inverted Microscope. Arabidopsis root was stained with DAPI blue dye.

Honey Bee

This photograph is of a wing of a honey bee under the Zeiss microscope using brightfield microscopy.
This is a DIC image of a diatom using the 60x oil immersion objective on the Nikon Inverted microscope. The long axis was oriented parallel to the analyzer.

hairy

40x hair from a bee under DIC with the Zeiss

Flower Pollen

Stamen and pollen of a yellow flower found on JMU campus. Image was taken using the Zeiss dissecting microscope using 3.2x objective magnification. Flower was picked in the middle of November and had yet started to die.

Tuesday, November 15, 2011

that's pretty sweet

This photograph of sugar crystals was taken with the Olympus using darkfield microscopy. The 4X objective lens was used and the exposure and sharpness were adjusted.

Root

This was taken on the confocal microscope at 2x (performed a z-stack and rendered).  Alexa 488 and DAPI were used on this Arabidopsis root. 

Sunday, November 13, 2011

BPAE


This image was taken using the confocal microscope. The BPAE cells were dyed with Texas red-X- phalloidin (Actin), BODIPY FL(microtubules) and Dapi(nuclei). When saved the blue and red were reversed making for an interesting picture!

Saturday, November 12, 2011

arabidopsis!

This teal root was taken under the 60x objective on the Nikon using confocal microscopy and is an overlay of blue and green dye staining.

alien invader

culex mouthparts under 4x with the zeiss

Friday, November 11, 2011


Fluorescence image of Bovine Pulmonary Arterial Endothelial celss (BPAE) at 40x objective taken on Nikon Fluorescence Microscopy. Cells were stained with DAPI, Texas Red and BODIPY.

Sugar Rush

This image of sucrose crystals was taken using widefield fluorescence microscopy on Nikon inverted microscope.  Images were taken on red, green, and blue emitting wavelengths of light.  The view field was then moved slightly, the images retaken with red, green and blue light, and the unaligned images overlaid on one another.  Four different versions of these images, including a monochrome image, were stitched together in Photoshop.

Buzz Buzz

Multichannel wide-field image taken of an autofluorescent adult fruit fly, with red, green, and blue filter blocks in place. A sharpen filter was applied to the image and LUTs were adjusted to make the colors brighter.

Hairy Lil' Fella


Hair Today, Hair Tomorrow
















These images of three different hair types were taken using phase microscopy at 20x.

Thursday, November 10, 2011

root tip

This fluorescence image of an Arabidopsis root tip was taken on the Nikon at 60X magnification using filter cube #1. Exposure was adjusted and sharpening tool was used.

Granite

This is a picture of a dirty whiteboard eraser.  It was taken
on the dissecting microscope at 2.5x.